Cloning and Sequence Analysis of the Isoflavone Reductase-like(FtIRL) Gene from Fagopyrum tatarium

The cDNA sequence of isoflavone reductase-like gene IRL from Fagopyrum tataricum was amplified by RT-PCR.Sequencing showed that the ORF of IRL gene from F.tataricum(named as FtIRL) was 942bp,probably encoding a protein of 313 amino acids.Further analysis indicated that the deduced FcIFR protein had homology values of 97% with other plants,IRLs.FtIRL possessed two short-chain dehydrogenase/reductase conserved motifs: a substrate-binding pocket and a NADPH binding site.The phylogenetic tree constructed based on amino acid sequences indicated that,although the amino acid sequence of IRL gene from F.tataricum had high homology with that of isoflavone reductase(IFR) genes from other plants,its biological function might be more similar to that of pinoresinol lariciresinol reductase(PLR).