Study on isolation of endophytic fungi from Sinopodophyllum hexandrum and their antimicrobial activities

Objective To explore the resource diversity of endophytic fungi from the endangered Sinopodophyllum hexandrum and its antimicrobial activity.Methods The endophytic fungi were isolated by tissue block method.Five pathogenic fungi and four bacteria were used as indicators to test the antimicrobial activity by agar plate antagonistic action and improved agar gel diffusion methods.Results The results indicated that 49 endophytic fungi strains were isolated from the roots,stems,and leaves of S.hexandrum,the most was from the roots(22),then from the stems(18),but the least was from the leaves(19);The isolated strains attributed to nine genera,three families,and two orders,and Fusidium sp.was the dominant genera based on morphological characters;For the isolated strains,22 of them expressed the inhibition to one or more pathogenic fungi,amounting to 44.8% of the total isolates,and eight strains were found to have significant inhibition against two or more pathogenic fungi,making up 16.3% of the total isolated strains,and three isolated endophytic fungal strains showed the evident inhibition against four or more indicating pathogenic fungi,and the two isolated strains showed the obvious antibacterial activity against the two indicating bacteria.Conclusion The endophytic fungi isolated from S.hexandrum have the diversity and evident inhibition against exterior fungi,active strains belong to the fungi of Aspergillus sp.,Hormodendrum sp.,and Fusidium sp.,respectively,and its research and development are of ecological and economic significances.