Isolation and Microbial Inhibition Activity of Endophytic Fungus from Cynanchum komarovii

To understand the diversity and microbial inhibition activities of endophytic fungi from desert plant,endophytic fungi were isolated from the root,stem and leaf of Cynanchum komarovii by tissue inoculation culture.The microbial inhibition activities of the endophytic fungi were tested using five pathogenic fungi strains and four pathogenic bacteria strains as indicators.Results indicated that twenty-five fungal endophytic strains were isolated and most of them were from stem.The isolated strains belonged to eleven genera,three families and two orders based on morphological characteristics.The 84.0%strains of total isolates had inhibitory activities against two or more tested fungal pathogens,and 60.0%strains had inhibitory activities against one or more tested pathogenic bacteria.Two strains were identified as Aspergillus,having obvious inhibitory activities against tested pathogens.The distribution of endophytes had significant difference among the root,stem and leaf of Cynanchum komarovii.The population of the isolates had the characteristics of diversity with different inhibition activities showing the abundant resources of pathogenic inhibition.The two strong active strains of Aspergillus had relatively broad inhibition spectrum.