Organ tissue culture of Lilium formolongi
Huang Min-ling
Abstract
Huang Min-ling
Abstract
The floral organ culture and rapid propagation of Lilium formolongi was studied by using its pedicel,receptacle,petal,filament and style for explants. The results showed that (a) adventitious buds could be induced directly from all of the 5 floral organs; (b) the appropriate medium was formulated with MS+2.0 mg 6-BA·L-1+0.2 mg NAA·L-1; (c) the order of inducing ability of the organs was:receptaclepedicelfilamentpetal or style; (d) the optimum medium formulation for adventitious buds proliferation was:MS+2.0 mg 6-BA·L-1+1.0mg KT·L-1+0.2 mg NAA·L-1; (e) the appropriate medium for in vitro root and bulb formations was:1/2MS+0.03 mg NAA·L-1; and (f) the most efficient and low-cost medium for transplantation was:1/4 (v/V) garden mould+1/4 (v/V) peat mould+1/2 (v/V) sawdust.
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The floral organ culture and rapid propagation of Lilium formolongi was studied by using its pedicel,receptacle,petal,filament and style for explants. The results showed that (a) adventitious buds could be induced directly from all of the 5 floral organs; (b) the appropriate medium was formulated with MS+2.0 mg 6-BA·L-1+0.2 mg NAA·L-1; (c) the order of inducing ability of the organs was:receptaclepedicelfilamentpetal or style; (d) the optimum medium formulation for adventitious buds proliferation was:MS+2.0 mg 6-BA·L-1+1.0mg KT·L-1+0.2 mg NAA·L-1; (e) the appropriate medium for in vitro root and bulb formations was:1/2MS+0.03 mg NAA·L-1; and (f) the most efficient and low-cost medium for transplantation was:1/4 (v/V) garden mould+1/4 (v/V) peat mould+1/2 (v/V) sawdust.
Key concepts: Explant culture, Bulb, Organ culture, Pedicel, Receptacle, Botany, Petal, Sawdust