2004•Chinese Journal of VeterinaryRequires access
Cloning and Sequencing of IL-18 cDNA from Chicken Embryo Spleen
Chunjie Zhang, Yinju Li, Wu Tingcai, Xiangchao Cheng
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Abstract
A pair of primers specific to chicken IL-18 were designed and synthesized according to the previously published sequence,to amplify chicken IL-18 cDNA by reverse transcription polymerase chain reaction(RT-PCR) from spleen cells of chicken embryo inoculated with Newcastle disease virus.Sequence analysis showed that the IL-18 cDNA was 594 bp in length,and encoded a protein of 198 amino acids.Compared with the chicken IL-18 cDNA reported by Schneider K,the homology of nucleotide acid and amino acid were 99.8% and 100%,respectively;the mature peptide gene homology of nucleotide and amino acid were 99.8% and 99.4%.This study is the first report of chicken IL-18 complete gene in China,it will lay a sound foundation for further study on the structure,function,expression of chicken IL-18.In addition,it also provide a new way for amplify chicken IL-18 and other cytokine.