Effects of Zhimaining on expressions of nuclear factor-κB and heat shock protein-70 in vascular endothelial cells-304 induced by cigarette smoke extract

Objective:To investigate the effects of Zhimaining in serum on the expressions of nuclear factor-κB (NF-κB) and heat shock protein-70 (HSP-70) in vascular endothelial cells-304 (VEC-304) induced by cigarette smoke extract (CSE). Methods: The expressions of NF-κB and HSP-70 in four cell groups: CSE group, group 1 (treated with high dose of Zhimaining), group 2 (treated with low dose of Zhimaining) and normal control group were test by immunohistochemical method. Results: The expressions of NF-κB [(288±25) vs. (185±14)]and HSP-70 [(370±28) vs. (258±20)]in CSE group were significantly higher than those of normal control group [P0.05~0.01], and the expressions of NF-κB [group1 (193±17), group2 (211±14)]and HSP-70 [group1 (262±21), group2 (282±23)]in the two groups treated with Zhimaining were significantly lower than those of CSE group [P0.05~0.01], and it′s more obvious in group 1. For cell DAB coloration test, NF-κB and HSP-70 showed brown-yellow or brown in CSE group, and they were significantly darker than those of normal control group. Though the colors in group1, 2 were darker than those of normal control group, but significantly lighter than those of CSE group(P0.05). Conclusion: Cigarette smoke extract could up-regulate the expressions of nuclear factor-κB and heat shock protein-70 in vascular endothelial cells-304, whereas, Zhimaining could down-regulate them.