2010•Zhongguo weishengtaixue zazhiRequires access
Screening and genotyping of metallo-β-lactamase in imipenem-resistant Pseudomonas aeruginosa.
Shan Sun, Liping Zhang
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Abstract
Objective To study the metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa(P.aeruginosa)isolated from our hospital.Method The imipenem-EDTA(IMP-EDTA)disc test was used to detect the metallo-β-Lactamase phenotypes and the PCR amplification method was used to detect the VIM and IMP genes.Result 5 strains of P.aeruginosa were positive for production of metallo-β-Lactamase by IMP-EDTA disc test with the sensitivity of 100% and specificity of 96%.4 strains of P.aeruginosa were positive using the PCR method.The PCR products were analyzed by DNA sequencing,which showed that all the positive strains were IMP-9 type.Conclusion The P.aeruginosa producing IMP-9 metallo-β-Lactamase of the 29 strains P.aeruginosa in our hospital is prevalent in our hospital and the IMP-EDTA disc test can screen the metallo-β-Lactamase positive P.aeruginosa simply and effectively.