LONG EFFECT OF IMMOBILIGED IFN-γ AND TNF-α ON APOPTOSIS OF HELA CELLS

The long-term inhibitory effect of co-immobilized IFN-γ and TNF-α on the growth of HeLa cells is investigated.IFN-γ and TNF-α were co-immobilized on cell culture polystyrene and cells were incubated 18d on that.Cell counting was used to determine the inhibitory rate of co-immobilized cytokines on HeLa cells.Inverted microscope was used to observe the cellular form.The analysis for the exposure of phosphatidylserine was used to testify the apoptosis.The study showed that the co-immobilized IFN-γ and TNF-α(20ng/well)could inhibit HeLa cells growth in a time-dependent manner,reached 94.12% inhibitory rate in 18 days and had significant activity of inducing HeLa cells apoptosis.The analysis for the exposure of PS showed that the apoptosis rates reached 76.6% and 88.7% after being cultured 3 and 6 days respectively.