Study on 20 β-lactamase genes and outer membrane protein oprD_2 gene in Pseudomonas aeruginosa isolated from burn department

OBJECTIVE To investigate the status of β-lactamase genes and outer membrane protein oprD2 gene in Pseudomonas aeruginosa(PAE) isolated from burn department. METHODS GNS-448 and K-B tests were performed to detect the susceptibility of 19 kinds of antimicrobial agents against 32 strains PA;β-lactamase genes and membrane pore-forming protein oprD2 gene were detected by polymerase chain reaction(PCR) and verified by DNA sequencer. RESULTS Resistant rates of 32 PA strains to ceftazidime,cefepime,cefoperazone,piperacillin,amikacin,gentamecin,imipenem and meropenem were 59.4%,56.3%,46.8%,62.5%,32%,43.7%,68.8% and 59.4%,respectively;The deletion ratio of oprD2 gene was 100.0%(32/32),The positive rate of TEM,OXA-10,VEB,IMP and VIM were 100.0%(32/32),28.1%(9),25.0%(8),25.0%(25) and 9.4%(3),respectively.The total positive rate of β-lactamase genes was 100.0%. CONCLUSION PAE isolates isolated from burn department are multdrug-resistant.oprD2 gene is absolute deletion,and the positive rates ofβ-lactamase genes are high,mainly including TEM,OXA-10,VEB,IMP and VIM.