Isolation,Identification & Drug Sensitivity Test of the Pathogenic Bacteria of Cow Mastitis

The mastitis of total 150 cows in Zhongyuan area was tested with the method of the method of CMT(California Mastitis Test) to investigate the situation of mastitis disease.The results showed that among 500 mammary areas at one dairy farm,the incidence of head numbers and mammary areas in sub-clinical mastitis was 68 % and 62.2 %,respectively.The isolation from milk of dairy cows with sub-clinical mastitis and the identification indicated the main microbial pathogens belonged to three species: Streptococcus,E.coli and Staphylococcus,the incidence of which was 39.6 %,27.1% and 15.6 %,respectively.The drug sensitivity test showed that all pathogenic bacteria were resistant to penicillin,streptomycin and sulfonamide after frequently used,while Ofloxacin,Kanamycin and Ciprofloxacin had a fairly good inhibiting effect on the pathogenic bacteria.