The POD Isozyme Analysis of Different Gastrodia elata Germplasms

The aim of the research was to discuss the genetic relationship of different Gastrodia elata germplasms cultivated in Lueyang county.The POD isozyme system in the scapes of three Gastrodia elata germplasm cultivated in Lueyang county was studied by means of vertical polyacrylamide gel electrophoresis.The results indicated that the three germplasm presented different peroxidase isozyme activities,and had their own key bands.Gastrodia elata Bl.f.elata,Gastrodia elata Bl.f.viridis Makino,Gastrodia elata Bl.var.obovata sp nov.respectively had nine,eight,and seven isozyme bands.The results also showed that Gastrodia elata Bl.var.obovata sp nov.was the earliest germplasm and Gastrodia elata Bl.f.elata was the most evolving germplasm in the three.POD zymogram belts of different germplasm had abundant polymorphism,which indicated that different populations of Gastrodia elata was distinguishing on the genetic essence.And the difference of the POD isozyme zymogram among different germplasms was obvious except for a few common characteristic zymogram belts.