2013•Liaoning zhongyi zazhiRequires access
Effects of Modified Xijiao Dihuang Decoction in Rabbit Model of Endotoxemia Induced by Lipopolysaccharide
Wenjuan Hu
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Abstract
Objective:To investigate the effects of promoting blood circulation and removing blood stasis on expressions of serum ET-1,vWF,TNF-α and CD62P levels and conjunctiva microcirculation disorders in rabbit model of endotoxemia induced by lipopolysaccharide.Methods:32 New Zealand white rabbits were randomized into 4 groups for blood-cooling group,blood-promoting group,model control group and normal control group.Treated with modified Xijiao Dihuang decoction and Taohong Siwu decoction respectively while modeling,serum CD62P was determined by flow cytometry and serum ET-1,vWF and TNF-α levels were determined by ELISA.Conjunctiva microcirculation changes were recorded.Results:At 3rd hour after injection of LPS,the levels of serum ET-1,vWF,CD62P and TNF-α were getting higher significantly in three groups than those in normal control group(P0.05,P0.01).At 3rd and 24th hour after injection,serum ET-1,vWF,TNF-α and CD62P levels and scores of microcirculation in blood-cooling group and ET-1,vWF,CD62P and scores of microcirculation in blood-promoting group were significantly lower than model control group(P0.05).Conclusion:The therapy of promoting blood circulation and removing blood stasis has efficacy of anti-inflammation,suppressing activation of platelet and protection against vascular endothelial injury.Modified Xijiao Dihuang decoction is more effective than Taohong Siwu decoction in rabbit model of endotoxemia induced by lipopolysaccharide.