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2011•Practical Preventive MedicineRequires access

Construction and Expression of pGEX-KG/TSARG1 Recombinant Vector in Rats

Gang Liu

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Abstract

Objective To construct pGEX-KG/TSARG1 recombinant vector in rats.Methods The open reading frame(ORF) of TSARG1 was amplified from rats' testis RNA by RT-PCR.The products were cloned into pUCm-T vectors and then sequenced.Then the recombinant plasmid was digested and subcloned into pGEX-KG vector.The recombinant plasmids were identified by DNA sequence analysis and transformed into component E.coli BL21 cells,the GST/ TSARG1 fusion protein was expressed with IPTG induction.Results The pGEX-KG/TSARG1 recombinant vector was successfully constructed.The GST/TSARG1 fusion protein was expressed abundantly after IPTG induction.Conclusions pGEX-KG/TSARG1 recombinant vector is constructed successfully,which may facilitate further investigation of the role of TSARG1 in spermatogenesis.

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What this paper is about

Objective To construct pGEX-KG/TSARG1 recombinant vector in rats.Methods The open reading frame(ORF) of TSARG1 was amplified from rats' testis RNA by RT-PCR.The products were cloned into pUCm-T vectors and then sequenced.Then the recombinant plasmid was digested and subcloned into pGEX-KG vector.The recombinant plasmids were identified by DNA sequence analysis and transformed into component E.coli BL21 cells,the GST/ TSARG1 fusion protein was expressed with IPTG induction.Results The pGEX-KG/TSARG1 recombinant vector was successfully constructed.The GST/TSARG1 fusion protein was expressed abundantly after IPTG induction.Conclusions pGEX-KG/TSARG1 recombinant vector is constructed successfully,which may facilitate further investigation of the role of TSARG1 in spermatogenesis.

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Available abstract

Objective To construct pGEX-KG/TSARG1 recombinant vector in rats.Methods The open reading frame(ORF) of TSARG1 was amplified from rats' testis RNA by RT-PCR.The products were cloned into pUCm-T vectors and then sequenced.Then the recombinant plasmid was digested and subcloned into pGEX-KG vector.The recombinant plasmids were identified by DNA sequence analysis and transformed into component E.coli BL21 cells,the GST/ TSARG1 fusion protein was expressed with IPTG induction.Results The pGEX-KG/TSARG1 recombinant vector was successfully constructed.The GST/TSARG1 fusion protein was expressed abundantly after IPTG induction.Conclusions pGEX-KG/TSARG1 recombinant vector is constructed successfully,which may facilitate further investigation of the role of TSARG1 in spermatogenesis.

Key concepts: Recombinant DNA, Molecular biology, lac operon, Biology, Fusion protein, Vector (molecular biology), Plasmid, Open reading frame

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Construction and Expression of pGEX-KG/TSARG1 Recombinant Vector in Rats — Research Paper | ScholarLens