Construction of Recombinant Plasmid of GST-hBLyS Fusion Protein Gene and Expression of GST-hBLyS Fusion Protein in E.coli BL21

The human B lymphocyte stimulator (hBLyS) gene, a 858 bp DNA fragment, was amplified by RT_PCR using a pair of primers which were designed and synthesized according to the hBLyS gene sequence in GenBank. The hBLyS gene was cloned into pGEX_4T_1 plasmid,a GST expression Vector. The recombinant expression plasmid of pGEX_4T_1/hBLyS was transformed into E coli BL21. The recombinant GST_hBLyS fusion protein was expressed after induced by IPTG in E coli BL21.