Preliminary Report on SCAR Maker for Distinguishing Allogynogenesis Grass Carp F_1 and Common Grass Carp

The genomic DNA from common grass carp (PC) and allogynogenesis grass carp F1 (CC) induced by inactivate sperms of squaliobarbus curriculus were isolated. Using RAPD and SCAR technology, 16 primers with high repeatability and stability were selected from the 100 RAPD random primers, and 7 specific DNA fragments had been discovered. One of the fragments was specific in allogynogenesis grass carp F1, while the others only appeared in common grass carp variety. After purification, cloning and sequencing, only two specific fragments amplified by S32 and S336 had been obtained. Based on their sequence information, SCAR primers were designed. However, the PCR products amplified by S336-Scar1F/S336-Scar1R were the same between two populations, and so were SCAR primers S336-Scar2F/S336-Scar2R. Fortunately, there was difference between common grass carp variety and allogynogenesis grass carp F1 varity when amplified by S32-Scar1F/S32-Scar1R and S32-Scar2F/S32-Scar2R. 115 individuals originated from 2 populations, including 55 CC and 60 PC, were used to verify the reliability of the SCAR maker S321643 (amplified by S32-Scar1F/S32-Scar1R). The results showed S321643 appeared in 46 PC individuals (76.7%), while disappeared in all 55 CC individuals (0%).