Synthesis and evaluation of a molecularly imprinted polymer microsphere for kaempferol

In order to effectively separate the active natural flavonoids kaempferol(KAE),a molecularly imprinted polymer(MIP)microsphere was prepared in acetonitrile using KAE,4-vinyl pyridine(4-VP)and ethylene glycol dimethacrylate(EDMA)as the template,functional monomer and cross-linker respectively.Afterwards,the synthesized MIP was analyzed by SEM,FTIR and TG.The parameters studied for kaempferol include adsorption kinetics and adsorption isotherm.The optimum synthesis conditions of the MIP microsphere were as follows:synthesis temperature was at 60℃,the ratio of functional monomer to solvent was 1∶20,and the ratio of template to functional monomer was 1∶8.The experimental results showed that the MIP exhibited a favorable affinity for kaempferol,and the saturated capacity could reach to 1.053 mg/g.The Scatchard analysis revealed that the specific adsorption of kaempferols existed in MIP.