Preparation and Application for Kaempferol Molecularly-imprinted Polymers

A molecularly-imprinted polymer(MIP) was prepared by bulk polymerization,using kaempferol as the template molecule,4-vinyl pyridine as the functional monomer,and ethylene glycol dimethacrylate as the cross-linker in the solvent system of CHCl3-DMF(3 ∶1,volume ratio).The synthesized polymer,i.e.kaempferol-MIP,was analyzed by FT-IR and scanning electron microscopey(SEM).In addition,the adsorption properties and mechanism were studied as well.The experimental results showed that the kaempferol-MIP exhibited a favorable affinity for kaempferol.The Scatchard analysis reveals that there exists a class of homogenous recognition sites in kaempferol-MIP within the range of certain concentration.Apparent maximal combination amount(Qmax) and equilibrium dissociation constant(KD) were calculated to be 3 938 μg/g and 9.074 mg/L,respectively.The result of selectivity study showed that the selectivity coefficient is 3.24 with respect to kaempferol-rutin system.