Clinical research on the changes of motilin and vasoactive intestinal peptide in gallstones with different distributions and properties

Objective To observe the changes of motilin(MTL) and vasoactive intestinal peptide(VIP) in gallstone with different distributions and properties and investigate the role of intestinal barrier function in gallstone formation. Methods One hurdred and eight patients with gallstones who should be operated in 363 Hospital from Mar. 2011 to Mar. 2013 were chosen,and there were 20 healthy controls. According to different distributions of gallstones,patients were divided into the normol control group(A1 group,n = 20),the gallbladder group(B1 group,n = 36),the bile duct group(C1 group,n = 36),and the combined group which were gallbladders complicated with bile ducts(D1 group,n = 36). After operation,gallstones were collected to analyzed by chemical process. On the basis of different properties of gallstones,the normol control group(A2 group,n = 20),the cholesterol stone group(B2 group,n = 40),the pigment stone group(C2 group,n = 52),and the combination calculus group which were cholesterol stones complicated with pigment stones(D2 group,n = 16) were chosen. MLT and VIP in blood and ileum mucosa were measured by radioimmunoassay. Results MLT and VIP both in blood and intestine showed higher levels in A1 group compared with B1,C1,D1 group(P 0. 05),but there was no significantly difference between B1 group and C1 group,as well as between C1 group and D1 group(P 0. 05). There were significant difference in A2 group compared with B2,C2,D2group(P 0. 05),respectively. There were significant difference in C2 group compaced with B2,D2 group(P 0. 05),respectively. There was no signifcant difference between B2 group and D2 group(P 0. 05). Conclusion The changes of gastrointestinal endocrine hormone can be found in pigment stones. There may be a correlation between pigment stone formation and the damage of intestinal mucosal barrier function. It is possible that this kind of intestinal injury play a role in the pigment stone formation.