Preparation and characterization of docetaxel nanoliposomes

Objective: OBJECTIVE To prepare docetaxel nanoliposomes and investigate their physico-chemical properties and release profile in vitro. Methods: Docetaxel liposomes were prepared by film dispersion-high pressure homogenization technology. The formulation was optimized using single factor screening. Transmission electron microscopy was used to study the shape. The diameter and Zeta potential was measured by dynamic light scattering. Retrodialysis method was used to determine the entrapment efficiency of the liposomes. In vitro drug release of the liposomes was investigated using dialysis method. Results: TS The optimized formulation was as follows: m( drug) ∶ m( lipids) = 1∶ 15,m( cholesterol) :m( soya lecithin) = 1 ∶ 8,organic phase / aqueous solution ratio was 5 ∶ 1. The mean diameter of the liposomes was( 200. 3 ± 3. 7) nm with Zeta potential of-15. 10 m V,and the entrapment efficiency was( 90. 76 ± 1. 42) ﹪. The in vitro drug release profile was fitted with Higuchi equation. Conclusion: The uniform nanoliposomes with high entrapment efficiency indicated the optimized formulation was feasible.