Effects of Bone Marrow Messchymal Stem cell Transplantaion Combined with Naomai Yihao on Angiogenesis in Cerebral Ischemic Tissue of Rat

Objective To observe the effects of bone marrow messchymal stem cell transplantaion,with Naomai yihao(NM),a compound traditional Chinese herbal medicine for regulating thesea of blood in brainon angiogenesis in cerebral ischemic tissue of rat. Methods A rat model of middle cerebral artery occlusion(MCAO) was established,and the rats were randomly divied into 3 groups:untreated group,BMSCs transplantation group,BMSCs transplantation+NMYH group.After 7-,14-and 21-day reperfusion,behavioral rating scale(BRS) of the rats and expression of VEGF in the brain tissue were measured by immunohistochemical method.Laser scanning confocal microscopy(LSCM) was used to measure angiogenesis. Results On 7、14、21days after cerebral ischemia,the neurological function of the rats between BMSCs transplantation group and BMSCs transplantation+NMYH group had significant recovery,the outcome of BMSCs transplantation+NMYH group surpassed the BMSCs transplantation group's(P﹤0.05).Compared with the model group,the numbers of VEGF positive cells in BMSCs transplantation group and BMSCs transplantation+NMYH group were increased significantly(P﹤0.01),the changes were better in BMSCs transplantation+NMYH group than in BMSCs transplantation group(P﹤0.05).Compared with the model group,diameter and area of microvessels significantly increased in all BMSCs transplantation group and BMSCs transplantation+NMYH group(P0.05),the changes were better in BMSCs transplantation+NMYH group than in BMSCs transplantation group(P﹤0.05). Conclusion The effect of improving the angiogenesis in boundary regions of ischemia and neurological function recovery in BMSCs transplantation+NMYH group was significantly superior to BMSCs transplantation group.BMSCs transplantation combined with NMYH has synergistic effects.The mechanism of improving angiogenesis may be correlated to the up-regulation of expression of VEGF.