Protective effect of bone marrow mesenchymal stem cells on hypoxic-ischemic brain damage in newborn rats

Objective To study therapeutic effect of intracerebral transplantation of bone mesenchymal stem cells(BMSCs)after hypoxic-ischemic brain damage in newbone rats.Methods SD rats were divided into control group,model group,and BMSCs transplantation group.HIBD model was made by ligating left side of the common carotid arteries of 7th day rat.The rats in BMSCs transplantation group received intracerebral transplantation of 1×105 BMSCs after 3 days hypoxia-ischemia.After 2 weeks and 4 weeks transplantation,animals were killed,and the brains were sectioned for HE staining and immunol fluorescence staining.Results After 4 weeks HE dyeing showed that nucleolis of control group were large,round and cytoplasmic rich.Cells of model group were necrosis,the neurons appeared empty.Compared with model group,cell of BMSCs transplantation group had less damaged,no empty bubble,and new generation capillary was visible.The apoptotic index was not differences between control groups(0.094±0.0003)and BMSCs transplantation groups(0.170±0.0002)(P0.05),but the apoptotic index of BMSCs transplantation groups was less than that of model group(P0.05).Conclusion BMSCs transplants can reduce hypoxic-ischemic brain damage of newborn rat neurons apoptosis index and promote neurological recovery in SD rats,curative the damage brain of ischemia oxygen.