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Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes

Xuetao Li

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Abstract

Objective:To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes.Method:The HPLC analysis was carried out on a ELITE C18(4.6 mm × 250 mm,5 μm) column with mobile phase consisted of acetonitrile-0.06 mol.L-1 KDP buffer(38∶ 62) at a flow rate of 1.0 mL.min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination.Result:The 1inear range of vinorelbine was 2.08 ～ 20.8 μg(r = 0.999 9).The average recovery was 98.64%(RSD 1.09%).The average entrapment efficiency was 86.83%.Conclusion:This method is simple,precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.

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Objective:To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes.Method:The HPLC analysis was carried out on a ELITE C18(4.6 mm × 250 mm,5 μm) column with mobile phase consisted of acetonitrile-0.06 mol.L-1 KDP buffer(38∶ 62) at a flow rate of 1.0 mL.min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination.Result:The 1inear range of vinorelbine was 2.08 ～ 20.8 μg(r = 0.999 9).The average recovery was 98.64%(RSD 1.09%).The average entrapment efficiency was 86.83%.Conclusion:This method is simple,precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.

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Available abstract

Objective:To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes.Method:The HPLC analysis was carried out on a ELITE C18(4.6 mm × 250 mm,5 μm) column with mobile phase consisted of acetonitrile-0.06 mol.L-1 KDP buffer(38∶ 62) at a flow rate of 1.0 mL.min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination.Result:The 1inear range of vinorelbine was 2.08 ～ 20.8 μg(r = 0.999 9).The average recovery was 98.64%(RSD 1.09%).The average entrapment efficiency was 86.83%.Conclusion:This method is simple,precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.

Key concepts: Entrapment, Vinorelbine, Chromatography, Liposome, Chemistry, High-performance liquid chromatography, Materials science, Surgery

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