2006•Zhongguo redai yixueRequires access
The expression of activated antigens in peripheral blood T lymphocyte subsets in patients with chronic active hepatitis B.
Hu Wang
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Abstract
Objective To investigate the expression of special marker for activation of peripheral blood T lymphocytes in patients with chronic hepatitis B virus and its significance. Methods Immunofluorescent three-color flow cytometry was used to study the expression of CD69 and HLA-DR on T lymphocytes in 30 patients with chronic active hepatitis B virus and 31 normal controls. The viral load was determined by fluorescence quantitative PCR. Results Expression of CD3+ T cells were significantly lower in patients(63.78%±9.70%)than that in controls (P0.01),meanwhile the expression of CD4+ T cells was 45.88%±8.51%,significantly lower than that of the controls (P0.001) and the expression of CD8+ T cell was 44.64%±7.10%,significantly higher than that of the controls (P0.001). Detection of CD69 on CD3+ T cells in patients was 6.85%±2.83%,significantly higher than that of the controls (P0.01). NO significant difference in the expression of CD69 on CD4+ T cells was observed between patients and the controls (P0.05),and the expression of CD69 on CD8+ T cells was 3.87%±1.81%,significantly different from that of the controls (P0.01). The expression of HLA-DR on CD3+ T cells were significance difference between patients (17.09%±4.23%)and that in controls (P0.001). The percentages of CD4+ T cells expressing HLA-DR in patients was 7.97%±2.43%,significantly higher than that of the controls (P0.01). Significant difference was observed in the expression of HLA-DR on CD8+ T cells in patients(5.52%±1.48%)as compared with controls (P0.001). There were positive correlations between percentages of CD8+ CD69+ ,CD8+ HLA-DR+ cells and viral load. Conclusion The findings support that cellular immunodeficiency exists in patients and that T lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8+ subset and it may play an important role in the immune response to antiviral infection.