Screening of Antagonistic Bacteria Against Botrytis cinerea and Identification of Strain X-75

In order to obtain antagonistic bacteria against Botrytis cinerea,about 200 strains of bacteria isolated from soil samples were screened with the agar plate diffusion method.After primary and secondary screening,the strain X-75 showed the most significant antagonistic activity.After the morphological observation and a series of physiological and biochemistry experiments,the bacterial strain X-75 was primarily identified as Bacillus sp.The similarity of the 16S rDNA sequences between X-75 strain and AB245422 Bacillus velezensis was 99.93%,so it was finally identified as the nearest genetic relationship with Bacillus velezensis.And the X-75 strain showed obvious antagonistic activity against Botrytis cinerea Pers on tomato leaves.