ScholarLens Back to search
2010•Journal of Xiamen UniversityRequires access

Exfoliazone Inhibits the Growth of HepG2 Cells by Inducing Apoptosis

Jin-Zhang Zeng

Open publisher page 0 citations

Abstract

This study is to investigate the anti-proliferation effect of Exfoliazone on liver cancer cell line HepG2 by inducing apoptosis.The test of anti-proliferation effect was performed by MTT assay,apoptotic cells were identified by DAPI staining and AnnexinV/PI staining.The expression of the poly ADP-ribose polymerase protein and its activated fragment were detected by Western-Blotting assays.Exfoliazone treatment significantly inhibited the growth of HepG2 cells in a time and dose-dependent manner.The IC50 for Exfoliazone was about 35,16 and 8 μmol/L after treatment for 24,48 and 72 h,respectively.Exfoliazone treatment could greatly induce apoptosis of HepG2 as indicated by DAPI staining,PARP cleavage and AnnexinV/PI staining.Exfoliazone inhibits the growth of HepG2 cells by inducing apoptosis.

About this research paper

What this paper is about

This study is to investigate the anti-proliferation effect of Exfoliazone on liver cancer cell line HepG2 by inducing apoptosis.The test of anti-proliferation effect was performed by MTT assay,apoptotic cells were identified by DAPI staining and AnnexinV/PI staining.The expression of the poly ADP-ribose polymerase protein and its activated fragment were detected by Western-Blotting assays.Exfoliazone treatment significantly inhibited the growth of HepG2 cells in a time and dose-dependent manner.The IC50 for Exfoliazone was about 35,16 and 8 μmol/L after treatment for 24,48 and 72 h,respectively.Exfoliazone treatment could greatly induce apoptosis of HepG2 as indicated by DAPI staining,PARP cleavage and AnnexinV/PI staining.Exfoliazone inhibits the growth of HepG2 cells by inducing apoptosis.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

This study is to investigate the anti-proliferation effect of Exfoliazone on liver cancer cell line HepG2 by inducing apoptosis.The test of anti-proliferation effect was performed by MTT assay,apoptotic cells were identified by DAPI staining and AnnexinV/PI staining.The expression of the poly ADP-ribose polymerase protein and its activated fragment were detected by Western-Blotting assays.Exfoliazone treatment significantly inhibited the growth of HepG2 cells in a time and dose-dependent manner.The IC50 for Exfoliazone was about 35,16 and 8 μmol/L after treatment for 24,48 and 72 h,respectively.Exfoliazone treatment could greatly induce apoptosis of HepG2 as indicated by DAPI staining,PARP cleavage and AnnexinV/PI staining.Exfoliazone inhibits the growth of HepG2 cells by inducing apoptosis.

Key concepts: DAPI, Apoptosis, Poly ADP ribose polymerase, Molecular biology, Staining, Blot, Cell growth, Pi

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Exfoliazone Inhibits the Growth of HepG2 Cells by Inducing Apoptosis — Research Paper | ScholarLens