2013•ZhongcaoyaoRequires access

ISSR analysis on genetic diversity of germplasms resources in Dendrobium SW. from different habitats

LU Jia-shi

Abstract

Objective To analyze the genetic diversity and genetic relationship of 24 plant samples in Dendrobium SW. from different habitats.Methods The molecular marker technique of inter-simple sequence repeat(ISSR) and unweighted pair-group method with arithmetic means(UPGMA) were used to study the genetic diversity and cluster analysis of 24 samples.Results Six primers with stable and clear diversity were sieved from 100 ISSR primers.DNA fragments(847) were amplified from 24 samples,and 141 DNA fragments were amplified from each primer in average,with 100% of polymorphic bands.Conclusion The 24 samples in plants of Dendrobium SW. from different habitats are divided into six groups and the genetic diversity is very abundant.

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Key concepts: UPGMA, Genetic diversity, Dendrobium, Biology, Primer (cosmetics), Microsatellite, Botany, Genetic variation