Influence of TaizijianII decoction on IL-8 and TNF-alpha in guinea pigs with allergic asthma

Objective To observe the effect of TaizijianⅡ decoction(TZJⅡ) on interleukin-8 (IL-8) and tumor necrosis factor(TNF)in asthma guinea pigs and to explore the mechanism of TZJⅡ on allergic asthma. Methods Fifty guinea pigs were randomly divided into five groups: control group, asthma model group, dexamethasone group, high-dose TZJⅡgroup and low-dose of TZJⅡgroup. All the guinea pigs were sensitized by injecting ovalbumin and induced asthma successfully, and then treatments were given to them for 15 days. Then weight changes were observed, and the incubation period of inducing asthma at last time were determinated. The lever of IL-8, TNFin bronchoalveolar lavage fluid (BALF) were measured by radioimmunity methods. Results TAmong the guinea pigs in model group,the symptoms of asthma were typical;the increasing of weightwas lower; the amounts of IL-8 and TNFin BALF were also higher ( 0.01). Compared with model group, guinea pigs in high dose TZJⅡgroup, the symptoms of asthma were relieved, and the weight was increased ( 0.05); moreover, the levers of IL-8 and TNF-alpha in BALF were lower( 0.05).Conclusions TZJⅡ can relieve the chronic allergic inflammation in air passage to some degree by regulating the network system of immunity function and inflammatory cytokine. The mechanisms of action of TZJⅡgroup are multiphase.