EFFECTS OF SOMATOSTATIN WITH GROWTH HORMONE ON LIVER INJURY IN DOG SAP

Objective: To study the change of the serum tumor necrosis factor α (TNFα) and their relations to liver damage in early severe acute pancreatitis (SAP) of dog model; to investigate the effect of somatostatin (SS) and growth hormone (GH) on dog SAP. Methods: 5% sodium taurocholate 2 mg/kg was injected into the main pancreatic duct to induce canine SAP model. Twenty dogs were randomly divided into four groups; Control group (n=5)、SAP model group (n=5)、 SS treatment group (n=5), SS and GH treatment group (n=5) in different phase. Serum levels of amylase (AMY), TNFα, albumin, ALT and AST were measured in 6 h, 12 h, 24 h, 48 h. In addition, histological changes of the pancreas and liver were examined by light and electron microscope in 48 hours after inducing SAP models. Those results are compared with control group. Results: The elevations of early serum TNFα, ALT and AST are correlated with the degree of liver damage. The application of SS and GH in different phase could decrease the serum AMY, TNFα and ease the liver's inflammation reaction and histopathologic damage. Conclusion: Appropriate application of SS and GH can inhibit AMY and SAP inflammatory mediators and pancreas liver inflammation, which prevents the development of SAP and protect the pancreas and liver from damage.