Screening of Isolating Medium for Wheat Endophytic Bacteria
Peng Juan
Abstract
Peng Juan
Abstract
Seven isolating media,including Beef extract Peptone Agar(BPA),Nutrient Agar(NA),Tryptic Soy Agar(TSA),Potato Dextrose Agar(PDA),Brain Heart Infusion(BHI),King's medium B(KMB),Luria-Bertani medium(LB) and Yeast extract Peptone Agar(YPA),were utilized to isolate endophytic bacteria from wheat,aiming at screening the optimal media.The results showed that the isolation effect of TSA and KMB was better than that of other media,while the PDA and BHI media had the lowest isolation effect.!The two media TSA and KMB were further confirmed to be the optimal media by using eight wheat varieties from eastern part of Henan province to isolate endophytic bacteria.
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Seven isolating media,including Beef extract Peptone Agar(BPA),Nutrient Agar(NA),Tryptic Soy Agar(TSA),Potato Dextrose Agar(PDA),Brain Heart Infusion(BHI),King's medium B(KMB),Luria-Bertani medium(LB) and Yeast extract Peptone Agar(YPA),were utilized to isolate endophytic bacteria from wheat,aiming at screening the optimal media.The results showed that the isolation effect of TSA and KMB was better than that of other media,while the PDA and BHI media had the lowest isolation effect.!The two media TSA and KMB were further confirmed to be the optimal media by using eight wheat varieties from eastern part of Henan province to isolate endophytic bacteria.
Key concepts: Agar, Isolation (microbiology), Potato dextrose agar, Bacteria, Nutrient agar, Food science, Yeast extract, Agar plate