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2006•Journal of ToxicologyRequires access

The effects of JNK RNA interference on 293 cell apoptosis induced by cadmium

Jiajun Wang

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Abstract

Objective To study the effects of JNK on 293 cell apoptosis induced by cadmium chloride(CdCl_2) with RNA interference.Methods Cells were incubated with CdCl_2 after siRNA transfection.The expressions of JNK and c-Jun were detected by rt-PCR and western blot,the rate of apoptosis was measured by flow cytometry(FCM) and the activities of cells were measured by MTT.Results The apoptosis rate of 293 cell treated with 30 μmol/L CdCl_2 was higher than those of the control group accompanied by increased expressions of JNK.siRNA-JNK could inhibit those changes but negative control and transfection agent couldn't.Conclusion The results suggested that JNK played a key role in 293 cells apoptosis induced by CdCl_2.

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What this paper is about

Objective To study the effects of JNK on 293 cell apoptosis induced by cadmium chloride(CdCl_2) with RNA interference.Methods Cells were incubated with CdCl_2 after siRNA transfection.The expressions of JNK and c-Jun were detected by rt-PCR and western blot,the rate of apoptosis was measured by flow cytometry(FCM) and the activities of cells were measured by MTT.Results The apoptosis rate of 293 cell treated with 30 μmol/L CdCl_2 was higher than those of the control group accompanied by increased expressions of JNK.siRNA-JNK could inhibit those changes but negative control and transfection agent couldn't.Conclusion The results suggested that JNK played a key role in 293 cells apoptosis induced by CdCl_2.

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Available abstract

Objective To study the effects of JNK on 293 cell apoptosis induced by cadmium chloride(CdCl_2) with RNA interference.Methods Cells were incubated with CdCl_2 after siRNA transfection.The expressions of JNK and c-Jun were detected by rt-PCR and western blot,the rate of apoptosis was measured by flow cytometry(FCM) and the activities of cells were measured by MTT.Results The apoptosis rate of 293 cell treated with 30 μmol/L CdCl_2 was higher than those of the control group accompanied by increased expressions of JNK.siRNA-JNK could inhibit those changes but negative control and transfection agent couldn't.Conclusion The results suggested that JNK played a key role in 293 cells apoptosis induced by CdCl_2.

Key concepts: Apoptosis, Transfection, Flow cytometry, Cadmium chloride, Chemistry, Western blot, RNA interference, Molecular biology

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