DNA microarray for detection of PRV,PPV and JEV

Detection microarray for PRV,PPV and JEV was investigated.A total of 16 target genes including PRV gB,gG,TK and gE,PRV NS2,NS3,VP1 and VP2 and JEV C,PrM/M,E,NS1 and NS5 were selected for preparation of PRV,PPV and JEV detection microarray.The target gene of 200 ng/μL were spotted on amino glass slides by microarray spotting system SpotArrayTM24.This detection system was highly specific and did not hybridize with DNA or cDNA of PRRSV,CSFV,PCV1,PCV2,TGEV,HPS,PAP,E.coli and S.pp.The microarray was able to detect as low as 3 pg/μL of target genes and was reproducible.The same microarray could be used for more than ten times.Specific hybridization spots were observed when tested against 8 PRV strains,5 PPV strains and JEV SA14-14-2 single or mixed samples.Test of 20 clinical samples by the PRV,PPV and JEV detection microarray showed a positive rate of PRV 15 %(3/20),PPV 5 %(1/20),JEV 0 %(0/20),PRV and PPV co-infection rate 15 %(3/20),PRV and JEV 5 %(1/20),JEV and PPV 5 %(1/20),PRV,PPV and JEV co-infection rate 5 %(1/20).The PRV,PPV and JEV detection microarray could differentiate PRV gene-deleted strains from wild type strains and is more sensitive than PRV,PPV and JEV multiple PCR.