Study on Transferring Insecticide Protein Gene from Bacillus Thuringensis into Maize Callus Via Agrobacterium Mediation

Factors influencing maize callus transformation mediated by Agrobacterium tumefaciens have been studied through gus transient expression and stable expression, such as different pre-cultivation time, different concentrations of AS and AgNO_3, different Agrobacterium growth phases, different infection time and infection media, et al. Maize embryogenic calli of inbred line 7922, H99 and P2 were transformed by Agrobacterium tumefaciens LBA4404(ACT). It was proved by PCR analysis that the target Bt. gene had been integrated into the genome of regenerated plants.