Optimization of Enzymatic Extraction Technology of SDF from Banana Peel by Response Surface Methodology

Response surface methodology is used to optimize the conditions for enzymatic extraction of soluble dietary fiber (SDF)from banana peel with the aim of maximizing the yield of SDF.The effects of enzyme concentration,treatment temperature,time and pH on the yield of SDF are studied in single factor experiments.Subsequently,a regres-sion model for the yield of SDF is established using a central composite design involving the above four factors at five levels and based on the model,response surface analysis is carried out to find out the optimum levels of these four factors.The results indicate that the optimum extraction conditions are enzyme concent-ration of 0.5%,treatment temperature of 49℃,time of 120 min and extraction pH of 5.3.Under these conditions,the experimental yield of SDF is 12.36%,which is basically consistent with the predicted one of 12.41%and increased by 9.47%than the maximum one of 30.51%obtained in single factor experiments.