Trichostatin A induces cell cycle arrest and apoptosis in bladder cancer T24 cells

Objective: To study the effect of trichostatin A (TSA) on inducing cell cycle arrest and apoptosis in bladder cancer T24 cells.Methods: T24 cells were exposed to varying concentrations of TSA (0.1μM,0.3μM and 1μM)for 24h.MTT assay was performed to evaluate cell viability.Apoptosis was analyzed with Annexin V-PI staining by flow cytometry.Active form of caspase-3 positive cells were measured by flow cytometry.Cell cycle distribution was analyzed by PI staining.Results: The results showed that TSA decreased cell viability in a dose dependent manner.TSA induced increased apoptotic cells evaluated by annexin V positive cells,which was accompanied with increased level of active caspase-3.TSA also induced G2/M arrest dose-dependently.Conclusions: These results suggest that TSA induces apoptosis by caspase-3 activation and promote G2/M cell cycle arrest.