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Genetic Diversity of High Quality Winter Wheat Varieties (Lines) Based on SSR Markers

Xin Chen, He Zhonghu, Shi Jianrong, Xia Lan-qin, R. G. Ward, Zhou Yang, Jiang GuoLiang

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Abstract

A set of 59 wheat SSR primers located on 21 wheat chromosomes were used to investigate the genetic diversity of 48 winter wheat cultivars and lines conferring good quality in winter and facultative region. A total of 209 allelic loci were detected, the number of alleles per primer pair ranged from 2 to 9, with average 3.5. The value of allelic polymorphism information content (PIC) ranged from 0.16 to 0.87, on an average, 0.56 per primer. The 48 wheat cultivars could be identified with combined 8 primers. Cluster analysis showed that all cultivars could be clustered into five groups, which is generally agree with pedigree analysis. The results indicated that SSR markers play an important role in variety identification and study of genetic diversity.

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What this paper is about

A set of 59 wheat SSR primers located on 21 wheat chromosomes were used to investigate the genetic diversity of 48 winter wheat cultivars and lines conferring good quality in winter and facultative region. A total of 209 allelic loci were detected, the number of alleles per primer pair ranged from 2 to 9, with average 3.5. The value of allelic polymorphism information content (PIC) ranged from 0.16 to 0.87, on an average, 0.56 per primer. The 48 wheat cultivars could be identified with combined 8 primers. Cluster analysis showed that all cultivars could be clustered into five groups, which is generally agree with pedigree analysis. The results indicated that SSR markers play an important role in variety identification and study of genetic diversity.

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Available abstract

A set of 59 wheat SSR primers located on 21 wheat chromosomes were used to investigate the genetic diversity of 48 winter wheat cultivars and lines conferring good quality in winter and facultative region. A total of 209 allelic loci were detected, the number of alleles per primer pair ranged from 2 to 9, with average 3.5. The value of allelic polymorphism information content (PIC) ranged from 0.16 to 0.87, on an average, 0.56 per primer. The 48 wheat cultivars could be identified with combined 8 primers. Cluster analysis showed that all cultivars could be clustered into five groups, which is generally agree with pedigree analysis. The results indicated that SSR markers play an important role in variety identification and study of genetic diversity.

Key concepts: Biology, Genetic diversity, Cultivar, Primer (cosmetics), Allele, Winter wheat, Microsatellite, Polymorphism (computer science)

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