Optimization of Polyphenol Extraction from Mango Kernel by Response Surface Methodology

Response surface methodology was applied for the optimization of process conditions for the extraction of polyphenols from mango kernel.Temperature,extraction time and solid-to-liquid ratio were identified as main factors that affect polyphenol extraction by means of one-factor-at-a-time experiments.Based on a 20-run,three-level Box-Benhnken experimental design and multiple regression analysis,a mathematical model equation was fitted that describes the extraction rate of polyphenols as a function of temperature,extraction time and solid-to-liquid ratio,and the relationship between the function and the three variables was analyzed using response surface analysis.As a result,the optimal extraction conditions were extraction temperature of 56 ℃,extraction time of 2 h,95% ethanol as extraction solvent with a solid-to-liquid ratio of 1:6.8.Under these conditions,the experimental extraction rate of polyphenols was 9.38%,which was close to the predicted value of 9.21% and obviously higher than the previously reported value of 6.86%.Therefore,the established extraction method has high reliability.