OPTIMIZATION OF EXTRACTION TECHNOLOGY OF POLYPHENOLS FROM MANGO PEEL

In the paper,we optimized the extraction technology of polyphenols from mango peel using response surface methodology.Selecting the ethanol volume fraction,the extraction time and the sodium hexameter phosphate amount as independent variables and the extraction amount as response value,we studied the effects of each independent variables and the interactive effects thereof on the extraction amount of polyphenols using Box-Behnken design,and carried out the regression analysis using Design Expert software.The results showed that the optimal polyphenol extraction conditions were as follows: ethanol volume fraction 41%,material-to-liquid ratio 1︰25,extraction temperature 65 ℃,extraction time 89 min,and sodium hexameter phosphate amount 0.19%,and the extraction amount of polyphenols reached 14.15 mg/g.The results of the verification test based on predictive model of polynomial quadratic equation showed that the actual extraction amount of polyphenols was 14.09 mg/g,which had little deviation from the predictive value.Accordingly,the optimal extraction technology optimized by the response surface methodology was reliable and accurate,and had practical value.