Investigation of contamination status of Pathogenic Vibrio isolated from seafood and Pearl River water in Guangzhou

Objective:To learn about the contamination status of pathogenic Vibrio isolated from seafood and Pearl River water in Guangzhou area and to provide scientific data for related health administration in making controlling measures.Methods: Vibrio cholerae were isolated and identified according to Diagnostic criteria for cholera WS 289-2008(Appendix A) and Mannual for cholera prevention and therapy.Vibrio parahaemolyticus were isolated and identified according to National Standards of Microbiological Examination for Vibrio parahaemolyticus(GB/T4789.7-2008).The isolation and identification of Vibrio vulnificus were performed according to National Surveillance Mannual for food-borne pathogenic bacteria.Results: 119 strains of pathogenic Vibrio were isolated from all 320 samples,with the detection rate being 37.19%.Among them,Vibrio parahaemolyticus had the most highest detection rate(26.56%),followed by Vibrio vulnificus(9.06%) and Vibrio cholera serogroup O1(1.56%).Significant differences were observed in the contamination rate among pathogenic Vibrio in different quarter of the year(P0.005).Conclusion: There is high degree of contamination by pathogenic Vibrio in seafood of Guangzhou area and it was advised by sanitary administrations to take effective measures to prevent food-borne diseases caused by pathogenic Vibrio from happening,especially in warm seasons.