Pathogenic vibrios in aquatic products and environment of Pearl River estuary

Objective To study the distribution of Vibrio cholerae and Vibrio parahaemolyticus(Vp)in different ecological environment of the Pearl River estuary in Guangzhou and to investigate their toxin genes.The relationship between Vibrio cholerae in the environment and the previous cases was also analyzed.Methods According to main characteristics of water network of Peal River estuary region in Guangzhou,1 608 samples of water,shellfish,fish,frog were collected.Toxin genes of the positive strain were detected by real-time PCR.Clustering analysis of strains isolated from previous cases in outbreak and outside environment of this study were carried on with PFGE.Results The total positive rate of Vibrio cholerae was 0.19%(3/1 608).No toxin gene was found.The total positive rate of Vp was 34.1% and the most of the strains did not carry toxin gene.Conclusion There are contaminations of Vibrio cholerae and Vibrio parahaemolyticus in aquatic products and environment of the Peal River estuary in Guangzhou.The highest rate of Vibrio parahaemolyticus was observed in shellfish.