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STUDY ON FERMENTATION CONDITIONS FOR PRODUCING ALKALINE CELLULASE BY BACILLUS SP. AC-2 STRAIN

Liming Xia

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Abstract

Fermentation conditions for producing alkaline cellulase by Bacillus sp.AC-2 were studied in shaking flasks strain.Results showed that the suitable inoculum age was 24 h.Optimal culture temperature and initial pH value were 37 ℃ and 10.5,respectively.The maximal alkaline cellulase activity reached 76.04 U/mL under the optimal conditions with 1% starch and 2% bean cake as carbon and nitrogen source,respectively.The enzyme preparations were stable between pH value 6.0 to 10.0 and below 70 ℃.The reaction catalyzed by alkaline cellulase at pH value 9.5 showed an optimum temperature of 60 ℃.This enzyme would have a good potential application in textile biofinishing and pulp-deinking.

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What this paper is about

Fermentation conditions for producing alkaline cellulase by Bacillus sp.AC-2 were studied in shaking flasks strain.Results showed that the suitable inoculum age was 24 h.Optimal culture temperature and initial pH value were 37 ℃ and 10.5,respectively.The maximal alkaline cellulase activity reached 76.04 U/mL under the optimal conditions with 1% starch and 2% bean cake as carbon and nitrogen source,respectively.The enzyme preparations were stable between pH value 6.0 to 10.0 and below 70 ℃.The reaction catalyzed by alkaline cellulase at pH value 9.5 showed an optimum temperature of 60 ℃.This enzyme would have a good potential application in textile biofinishing and pulp-deinking.

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Available abstract

Fermentation conditions for producing alkaline cellulase by Bacillus sp.AC-2 were studied in shaking flasks strain.Results showed that the suitable inoculum age was 24 h.Optimal culture temperature and initial pH value were 37 ℃ and 10.5,respectively.The maximal alkaline cellulase activity reached 76.04 U/mL under the optimal conditions with 1% starch and 2% bean cake as carbon and nitrogen source,respectively.The enzyme preparations were stable between pH value 6.0 to 10.0 and below 70 ℃.The reaction catalyzed by alkaline cellulase at pH value 9.5 showed an optimum temperature of 60 ℃.This enzyme would have a good potential application in textile biofinishing and pulp-deinking.

Key concepts: Cellulase, Chemistry, Fermentation, Erlenmeyer flask, Food science, Pulp (tooth), Laboratory flask, Strain (injury)

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STUDY ON FERMENTATION CONDITIONS FOR PRODUCING ALKALINE CELLULASE BY BACILLUS SP. AC-2 STRAIN — Research Paper | ScholarLens