Correlation between serum MMP-1,TIMP-1 and liver pathology in patients with chronic hepatitis B

Objective To investigate the relationship of serum matrixmetalloproteinase-1(MMP-1),tissue inhibitor of metalloprotienase-1(TIMP-1)with the severity of liver fibrosis and inflammation.To look for the index sign of serology of the new judgment of hepatic fibrosis degree.Methods Liver pathology was performed in 64 patients with chronic hepatitis B(CHB);hepatic histological findings were diagnosed by liver inflammation grades(G)and fibrosis stages(S);serum levels of MMP-1,TIMP-1 were detected by sandwich enzyme-linked immunosorbent assay(ELISA);serum levels of hyaluronidase(HA),Laminin(LN),collagen-Ⅳ(C-Ⅳ)and procollgen tpyeⅢ peptide(PⅢNP)were detected by radioimmunoassay.Results With the development of liver inflammation grades and fibrosis stages,the serum levels of MMP-1 gradually reduced,but the serum levels of TIMP-1 increased gradually;besides,there were no significant differences between the G1 and G2,the S1 and S2,the rest groups comparison had significant differences;serum TIMP-1 was correlated positively with the fibrosis stages(P=0.003)and inflammation grades(P=0.005).However,serum MMP-1 was correlated negatively with the fibrosis stages(P=0.004)and inflammation grades(P=0.007);serum MMP-1,TIMP-1 had good correlation with serum HA,PⅢNP.Conclusion Serum MMP-1 and TIMP-1 levels can be used as fibrosis markers.