Primary Investigation on MyoG Gene in Jinhua Pig
Jin Zhao
Abstract
Jin Zhao
Abstract
The genotypes of myogenin gene(at second intron and at 3' end)for 249 Jinhua pigs(line I,178;line II, 30;line III,41) were detected by PCR-RFLP. There were three genotypes of AA,AB and BB in the PCR1- MspⅠ-RFLP site,their frequencies were 0.154 4、0.382 6、0.463 1 respectively; Genotypes only were MM and MN in PCR2- MspⅠ-RFLP site,and the frequencies were 0.995 3 and 0.004 7.
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The genotypes of myogenin gene(at second intron and at 3' end)for 249 Jinhua pigs(line I,178;line II, 30;line III,41) were detected by PCR-RFLP. There were three genotypes of AA,AB and BB in the PCR1- MspⅠ-RFLP site,their frequencies were 0.154 4、0.382 6、0.463 1 respectively; Genotypes only were MM and MN in PCR2- MspⅠ-RFLP site,and the frequencies were 0.995 3 and 0.004 7.
Key concepts: Restriction fragment length polymorphism, Genotype, Myogenin, Genetics, Molecular biology, Gene, Intron, Genotype frequency