Study on the Polymorphism of Porcine Heart Fatty Acid-Binding Protein Gene in Eleven Pig Breeds Using PCR-RFLP

The genetic variation in 5′upstream (HinfⅠRFLP) and in intron 2 (HinfⅠRFLP?HaeⅢRFLP) of the porcine heart fatty acidbinding protein (HFABP) gene were detected with PCRRFLP in 420 pigs including Wuzhishan pig,Yimeng black pig,Hanjiang black pig,Laiwu pig,Xiang pig,Min pig,Chenghua pig,Neijiang pig,Erhualian pig,Bama xiang pig and Large White pig.The results suggested:(1)All the breeds varied at the HinfⅠRFLP site of the 5′upstream while Yimeng black pig and Large White only had 2 genotypes (HH?Hh).(2)At the HinfⅠRFLP site of the second intron,Erhualian only presented BB genotype while other breeds varied.(3)At the HaeⅢRFLP site of the second intron,the polymorphic site was only found in Wuzhishan,Xiang pig and Large White,D allele frequency was 086,098 and 068,respectively,other 8 breeds only showed DD genotype.