Expression and significance of C-kit gene in children with aplastic anemia

Objective To investigate the relationship between C-kit gene and mechanisms of aplastic anemia in children.Methods By using immunohistochemical staining and in situ hybridization techniques, the expressions of C-kit and C-kit mRNA in children with aplastic anemia were detected in 38 chronic aplastic anemia (CAA), 36 severe aplastic anemia (SAA) and 35 healthy controls.Results The immunohistochemistry results showed among CAA,SAA and control groups were 0.52±8.81,0.46±2.18,0.58± 7.94,and there was no significant difference among them(P0.05). The expressions of C-kit mRNA in CAA(0.47±6.02) and SAA (0.77±8.09) were the same as that of normal control (0.61±3.71).Hemopoietic stem cells of children with aplastic anemia expressed no more C-kit and C-kit mRNA than those of normal controls(P0.05).Conclusion Aplastic anemia was not attributable to low expressions of C-kit and C-kit mRNA on the hematopoietic stem cells.