Expression of proliferation cell nuclear antigen ki-67 in ectopic endometrium and eutopic endometrium

Objective:To explore the expression of proliferation cell nuclear antigen ki-67 in endometriosis and the effect of proliferative activity of endometrial cells on the occurrence of endometriosis.Methods:Immunohistochemical SP method was used to detect the expression levels of ki-67 in ectopic endometrium and eutopic endometrium of 58 cases with endometriosis(endometriosis group,including 36 cases with ovarian endometriosis and 22 cases with adenomyosis) and normal endometrium of 15 cases with hysteromyoma(control group).Proliferation index(PI) was counted as statistical index.Results:There was no significant difference in the expression level of ki-67 in ectopic endometrium and eutopic endometrium between ovarian endometriosis group and adenomyosis group;in control group and endometriosis group,the expression levels of ki-67 in gland and mesenchyma of eutopic endometrium at proliferative phase were significantly higher than those at secretory phase;in endometriosis group,there was no significant difference in PI of ki-67 in ectopic endometrium between proliferative phase and secretory phase(P0.05).In endometriosis group,at proliferative phase,PI of ki-67 in ectopic endometrium was lower than that in eutopic endometrium;but at secretory phase,PI of ki-67 in ectopic endometrium was significantly higher than that in eutopic endometrium(P0.01).In endometriosis group,the expression level of ki-67 in gland of ectopic endometrium of stage Ⅰ~Ⅱ was significantly higher than that in gland of ectopic endometrium of stage Ⅲ and stage Ⅳ(P0.05),but there was no significant difference in the expression level of ki-67 in eutopic endometrium of different clinical stages(P0.05).Conclusion:PI of ki-67 reflects the proliferative activity of endometrial cells,continuous proliferation and activation of ectopic endometrial cells in cases with endometriosis may play an important role in the occurrence and development of endometriosis.