Effect of Yuxianling Granules on the NGF of Hippocampus and Cognitive Function in Rats with Epilepsy Induced by Pentylenetetrazol

Objective:To study the effect of Yuxianling granule(YXLG) on the hippocampal nerve growth factor(NGF) and cognitive function in epileptic rats.Method:150 SD rats were used for the experiment,and 10 of them were randomly selected as the blank control group.The rest animals were treated with pentylenetetrazol(PTZ) to establish the model.The rats consisting with the model standard were divided randomly into 4 groups,including model group,YXLG group,sodium valproate group and lamotrigine group..Using gavage administration,the drug doses were 810 mg.kg-1,122 mg.kg-1,10.1 mg.kg-1 accordingly.The model group and control group were given the same volume of distilled water daily.All the treatment was 1 times daily,lasted for 28 d.The animals weretested by open field test for measuring changes in cognitive function.Immunohistochemical method was employed to observe the expression of NGF.Result:Compared with the model group,YXLG could increase the opening test results in rats with pentylenetetrazol-induced epilepsy,the difference was statistically significant(P 0.01).YXLG could enhance the average gray value of NGF-positive cells and reduce the average optical density of NGF-positive cells,the difference was statistically significant(P 0.01).Conclusion:The reason that YXLG improves the cognitive function of the epileptic rats may be related with the reduced expression of NGF in epileptic rats.