Effct of Yuxianling granule on cognitive dysfunction and ultrastructure of hippocampus CA3 area in PTZ-induced epileptic rats

Objective To investigate the effect of Yuxianling granules(YXL)on epileptic hippocampus CA3 area ultrastructure and cognitive function.Methods The epilepsy rat models,induced by intraperitoneal injection of pentylenetetrazol,were randomly divided into model(mode1),Yuxianling granules intervention,valproate intervention,lamotrigine intervention,and control groups;the learning and memory ability of rats were tested with experimental platform,and the ultrastructure of hippocampal CA3 area with transmission electron microscopy observation.Results Compared with normal and other groups,the learning function in the reaction period after modeling was significant(P0.01),but in incubation and memory score was no signif-icant(P0.05);after anti-epileptic intervention in all the groups,the differences of reaction period and incubation,and learning and memory score were significant(P0.001),the difference between Yuxianling group and normal group was no significant(P0.05),in Yuxianling and lamotrigine groups,they were superior than that insodium valproate and model groups(P0.05);before and after the anti-epileptic drug intervention,the reaction period,the incubation period,learning and memory score in Yuxianling group were better than them in lamotrigine group(P0.01),the dif-ference between sodium valproate and model groups was no significant(P0.05),the normal reac-tion period,latency,memory score were no significant(P0.05),but learning score was significant(P 0.01).With the electron microscope,hippocampal CA3 neurons was obviously injuryed in model group,the majority of neurons structure in Yuxianling group were normal,and minority of them in rough endoplasmic reticulum existed mild degranulation.Conclusions Lowering pentylenetetrazol kindling hippocampus neuronal necrosis in epilepsy rats is one mechanisms of Yuxianling to improve the cognitive dysfunction.