Determination of preservatives in soysauce by solid phase extraction-high performance liquid chromatograph

To develop a method for determination of 10 preservatives in soy sauce by solid phase extraction-high performance liquid chromatography(SPE-HPLC) simultaneously.Sample was extracted by 90% methanol solution,the extract was cleaned by PEP-SPE.Separation was achieved by the use of Plus C18 column(5 μm,250 mm×4.6 mm i.d),acetonitrile-0.01 mol/L potassium dihydrogen phosphate solution(pH=4.0) as mobile phase,a flow rate of 1.0 mL/min and the detector at 230 nm and 254 nm was used.The column temperature is 30 ℃,and the injection volume is 10 μL.The limit of detection is 1.0～2.0 mg/L.The linear plots were obtained between 1.0～100.0 mg/L.Overall recoveries were between 72.8%～100.9%,and RSD(n=4) value were 0.42%～6.87%.This method is simple,rapid,sensitive and effective for simultaneously determining 10 preservatives in soy sauce.