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Determination of benzoic acid in soy sauce by HPLC

Zhou Chun-hong

Open publisher page 1 citations

Abstract

A novel method is proposed for the separation and determination of benzoic acid (BA) in soy sauce by solid phase extraction and high performance liquid chromatography(HPLC). BA in soy sauce can be enriched by solid phase extraction with ENVI-18 cartridge and separated on a lrregular C18 column(4.6mmX20cm,10祄). The mobile phase of methanol/ammonium acetate(5:95)by linear equal elution at a flow-rate 1.00mL/min and 230nm the maximum absorption wavelength of detector were used; The separation was achieved within 6min. The average recoveries for real samples ranged from 95.8%~106.2%, the accuracy is 0.00104, and the RSD are 0.31%, the detection limits are g/L level. It is a simple and rapid method with good reproducibility. In future the method can be extended and used in monitoring works.

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What this paper is about

A novel method is proposed for the separation and determination of benzoic acid (BA) in soy sauce by solid phase extraction and high performance liquid chromatography(HPLC). BA in soy sauce can be enriched by solid phase extraction with ENVI-18 cartridge and separated on a lrregular C18 column(4.6mmX20cm,10祄). The mobile phase of methanol/ammonium acetate(5:95)by linear equal elution at a flow-rate 1.00mL/min and 230nm the maximum absorption wavelength of detector were used; The separation was achieved within 6min. The average recoveries for real samples ranged from 95.8%~106.2%, the accuracy is 0.00104, and the RSD are 0.31%, the detection limits are g/L level. It is a simple and rapid method with good reproducibility. In future the method can be extended and used in monitoring works.

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Available abstract

A novel method is proposed for the separation and determination of benzoic acid (BA) in soy sauce by solid phase extraction and high performance liquid chromatography(HPLC). BA in soy sauce can be enriched by solid phase extraction with ENVI-18 cartridge and separated on a lrregular C18 column(4.6mmX20cm,10祄). The mobile phase of methanol/ammonium acetate(5:95)by linear equal elution at a flow-rate 1.00mL/min and 230nm the maximum absorption wavelength of detector were used; The separation was achieved within 6min. The average recoveries for real samples ranged from 95.8%~106.2%, the accuracy is 0.00104, and the RSD are 0.31%, the detection limits are g/L level. It is a simple and rapid method with good reproducibility. In future the method can be extended and used in monitoring works.

Key concepts: Chromatography, Ammonium acetate, Chemistry, High-performance liquid chromatography, Benzoic acid, Cartridge, Solid phase extraction, Extraction (chemistry)

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