Speciation of arsenic compounds in plant seafood by atomic fluorescence spectometry

Objective:To establish a method for the determination of four arsenic species by the combination of Ion exchange chromatography and hydride generation atomic fluorescence spectrometry.Methods: In this research,an on-line system of HPLC and hydride generation atomic fluorescence spectrometry was used for arsenic speciation.It has realized on-line separation and analysis of four arsenic species(AsⅢ,DMAⅤ,MMAⅤ and AsⅤ).Results: Various experimental parameters were optimized.Four arsenic species were separated by PRX-X100 anion exchange column and determined within 12 minutes in optimal conditions.Under the hydride reaction condition of 8%HCl and 2%(m/V)KBH4 with 100 μl injection volume,the detection limits of various forms were 0.48 μg/L for As(Ⅲ),0.56 μg/L for DMA,0.44 μg/L for MMA and 0.52 μg/L for As(V).The precision RSD of various forms was 5%(n=7).The linear relationship was good in the range of 5 μg/L～100 μg/L content of various forms(r0.9992).Conclusion: This method is simple,convenient,rapid and accurate with high sensitivity and precision.It was suitable for speciation of arsenic compounds in plant seafood